Huaan Magnech Bio-Tech Co., Ltd.

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Chloramphenicol ELISA test kit

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Product Details:

Brand Name Magnech
Model Number 96T
Place of Origin Beijing, China (Mainland)

Payment & Shipping Terms:

Minimum Order Quantity: 1 Bag/Bags
Port: Tianjin
Delivery Time: 16days
Payment Terms: L/C,T/T,Western Union,MoneyGram
Supply Ability: 100000 Bag/Bags per Year

Detailed Product Description


 

Product code

HE09001

Specification

96 wells/kit  (Removable 8 wells x 12 strips)

Sensitivity

0.05ppb

Scope of application

Can be qualitative, quantitative detection of animal tissues, milk, honey, feed, eggs, etc. of chloramphenicol residues in samples

Introduction

Chloramphenicol (Chloramphenicol, CAP) was once widely used antibiotics, but because of its serious side effects, can cause human aplastic anemia, agranulocytosis Huntington's diseases, Europe and the United States and other developed countries have banned or strictly prohibit the use of successively .
    The ELISA kit is the application technology research and development of a new generation of drug residues in products, operating time was only 60min, to minimize operator error and the intensity of work.

Detection limit:

Egg  0.05 ppb

Honey  0.05 ppb

Milk  0.05 ppb

Tissue  0.025 ppb

Feed   0.2 ppb

Cross-reaction rate:

Chloramphenicol ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... .. ... ... ... ... ... 100%
Chloramphenicol base ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... 0.5%
Methyl Chloramphenicol ... ... ... ... ... ... ... ... ... ... ... .... ... ... ... ... ≤ 0.05%
 Florfenicol ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... "0.01%
Florfenicol amine ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... "0.1%
Tetracycline, gentamicin, and ampicillin did not show any cross-reactivity.

Recovery rate

Chicken, pork, fish, shrimp 102±15%

Honey  80±10%

Milk  110±10%

Egg   95±15%

Principle

Chloramphenicol (CAP) ELISA test Kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the tissue (chicken,pork), honey, milk, fish, shrimp and egg. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against Chloramphenicol. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Chloramphenicol in it. This value is compared to the standard curve and the content of the corresponding Chloramphenicol is subsequently obtained.

Storage methods and durability

12 Months when properly stored at 2~8 centigrade

Chloramphenicol ELISA test kit Chloramphenicol ELISA test kit

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